| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Brown |
A | Gonzalez | Ofek | Patil | Stimson | 4:05.00 | |
| 2 | Holy Cross |
B | Rhoden | Waldron | O'Sullivan | Marjanski | 3:57.00 | |
| 3 | Holy Cross |
C | Doherty | Murray | Whelan | Jordan | 4:00.00 | |
| 4 | Holy Cross |
A | Cummings | Murphy | Ronan | Gill | 3:53.42 | 2022 Boston University David Hemery Valentine Invitational |
| 5 | Bryant |
A | Barrows | Bertarelli | Haddad | Pineda | 3:46.66 | Penn Relays |
| 6 | Bryant |
B | Crowley | Grzybinski | Kamperides | Stygar | 4:19.00 |