| @ | Dragone Track | 48.53 | Springfield College | Springfield | 4/3/2010 |
| # | Middlebury Outdoor | 48.53 | Middlebury | Middlebury | 4/23/2022 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Middlebury |
A | White | Louie | Kaiden | Barns | 47.69 | 2023 Carla Coffey Invitational |
| 4 | A | Moncion-Allen | Baker | Stanton | Froe | 51.22 | RPI Under the Lights Invite 2023 | |
| 5 | NVU-Johnson |
A | Atuahene | Wooster | Buonanno | Eames | 55.00 | |
| 6 | Middlebury |
B | Allen | Gay | Belsher | Johnson | DQ | |
| 7 | Middlebury |
C | Pollitt | Lawrence | Bruno | Egwuonwu | NT |