| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | St Johnsbury | A | Dunn | Perez | Krebs | Vera | NT | |
| 2 | South Burlington | A | Blanchard | Poirier | Zuchman | Lambert | NT | |
| 3 | Bellows Free Academy - St. A | A | Auer | Hunsicker | Holland | Marras Gimenez | 2:14.56 | |
| 4 | Peoples Academy | A | Cleary | Crawley | Beal | Norsworthy | NT | |
| 5 | Missisquoi Valley Union | A | Bourdeau | Casperson | Favreau | Gregory | NT | |
| 6 | Mt Mansfield Union | A | NT |