| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Missisquoi Valley Union | A | Anderson | Gagne | Mayhew | Patterson | NT | |
| 2 | St Johnsbury | A | Strohm | Windrow | Lawton | Demas | NT | |
| 3 | Bellows Free Academy - St. A | A | Sweeney | Hurteau | Menard | Brace | 4:00.34 | |
| 4 | South Burlington | A | Lawrence | Fisher | Simone | Poquette | NT | |
| 5 | Peoples Academy | A | Forrest | Masten | Manning | West | NT |