| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | St Johnsbury | A | Thomas | Solana | Hatch | Thornton-Sherman | 3:38.00 | |
| 2 | Mt Mansfield Union | D | Cleary | Jacobs | Connell | Caforia-Magee | 4:02.00 | |
| 3 | Oxbow | A | Morse | Danis | Haley | Barrett | NT | |
| 4 | Burr And Burton Academy | A | Smith | Cavagnino | Addington | Kopeck | NT | |
| 5 | Thetford Academy | A | Mann | Sloop | Thaxton | Terry | NT | |
| 6 | Vergennes Union | A | McGuire | Hatch | Neffinger | Fearon | NT | |
| 7 | Rice Memorial | A | McKay | Wood | Washburn | Bretton | NT | |
| 8 | Rice Memorial | B | Hines | Kulapin | Mulcahey | Sotomonte-Hammond | NT | |
| 9 | Champlain Valley Union | A | Donahue | Latka | Meisenzahl | Snyder | NT | |
| 10 | St Johnsbury | B | Stocker | Thomas | O'Farrell | Pearson | NT | |
| 11 | Springfield | A | Stagner | Gould | Foley | Palmer | NT | |
| 12 | Mt Mansfield Union | A | Gilbert | Gilbert | Ryser | Desautels | NT | |
| 13 | Mt Mansfield Union | B | Glanz | Dumas | Moulton | Burnell | NT | |
| 14 | Mt Mansfield Union | C | Rodriguez | Maciejowski | Joyce | Stevens | NT | |
| 15 | North Country Union | A | Esposito | Isabelle | Mutter | Brown | NT | |
| 16 | Richford | A | Bean | Chagnon | Scott | Goodroe | NT |