| Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | |
|---|---|---|---|---|---|---|---|---|
| 1 | St Johnsbury | A | Ebbett | Rousseau | Hatch | Thornton-Sherman | 1:38.00 | |
| 2 | Champlain Valley Union | A | Edlebaum | Fagnant | Kogut | Wolny | 1:39.90 | |
| 3 | Essex | A | Mattos | Botelho | Hughes | Meek | 1:40.00 | |
| 4 | Rice Memorial | A | McKay | Jeonghun | Washburn | Bretton | 1:43.00 | |
| 5 | South Burlington | A | Chen | Foster | Sweenor | Lai | 1:50.00 | |
| 6 | Spaulding | A | Bradley | Pope | Renzello | Saldi | NT | |
| 7 | Lake Region Union | A | Badertscher | Forsay | Wilson | Lahar | NT | |
| 8 | Burr And Burton Academy | A | Perry | Cassan | Cavagnino | Gilliam | NT | |
| 9 | Thetford Academy | A | Junker | Terry | Perdrizet | Rich | NT | |
| 10 | Mt Abraham | A | Senecal | Sturtevant | Halby | Sawyer | NT | |
| 11 | South Burlington | B | Horton | Lai | Burt | Harraz | NT | |
| 12 | Rice Memorial | B | Papayiannis | Wood | Mulvey | Kulapin | NT | |
| 13 | Champlain Valley Union | B | Gordon | Barton | Pagnucco | Rafuse | NT | |
| 14 | St Johnsbury | B | Garand | Lemieux | Emmons | Carter | NT | |
| 15 | White River Valley | A | Armstrong | Bangerter | Mabey | Hughes | NT | |
| 16 | Colchester | A | Currie | Faas | Thomason | Langer | NT | |
| 17 | Essex | B | Bartlett-brown | Ackerman | Callahan | Wargo | NT | |
| 18 | UThirtyTwo | A | Frostick | Pearson | Farrell | Parker | NT | |
| 19 | Burlington | A | Fanslow | Foster | Snyder | Paschel-Harbour | NT | |
| 20 | Missisquoi Valley Union | A | Godin | Hubbard | Pilbin | Nelson | NT | |
| 21 | Mt Mansfield Union | A | Burnell | Caforia-Magee | Moulton | Glanz | NT | |
| 22 | Mt Mansfield Union | B | DeGruttola | Joyce | Dishman | Stevens | NT | |
| 23 | North Country Union | A | Mutter | Thompson | Isabelle | Provost | NT |